How to efficiently achieve real-time synchronous control experiments of live cells?

In general, when doing cell experiments, an experiment will be divided into an experimental group and a control group. The experimental group is the object group that receives the treatment of experimental variables; Compared with the experimental group, the control group is the object group that does not receive the treatment of experimental variables.
Theoretically, because the experimental group and the control group are affected by the same unrelated variables, the difference between the experimental group and the control group can be recognized as the effect from the experimental variable. Experimenters strengthen the credibility of experimental results by setting up experimental groups and control groups, which requires that experiments be carried out in as many groups as possible, synchronously, and corresponding data can be retained.

The effects of morphine, sufentanil and butorphanol on the proliferation of human lung cancer cell A549 cell lines were detected by cloning experiments, and A549 cells were treated with different concentrations of morphine, sufentanil and butorphanol for 7 days, and cell cloning experiments were performed.

The experimental results showed that when the morphine concentration was 101umol/L, the proliferation of A549 cells was inhibited, and the number of colony units was significantly lower than that of the control group, and the results were statistically significant. Other concentrations of morphine have little effect on the proliferation of A549 cells. Different concentrations of sufentanil and butorphanol showed negative results, that is, different concentrations of sufentanil and butorphanol did not have a significant effect on the proliferation of A549 cells.

2. Experiment on the effect of the same drug on different kinds of cells.

To compare the rate of inhibition of sorafenib on the proliferation of different cloned cells, different cells were cultured into clonal cell populations. Negative control wells are added with medium only, and cells adheres after 24 h. The positive control group and the negative control group did not add the drug sorafenib, and the experimental group added different concentrations of the drug sorafenib.

Clone A (SCLC-1) cells are small, the most numerous, and the structure is dense; Clones are large, with smooth and well-defined boundaries, and densely arranged cells in a nest-like shape, accounting for about 13% (2/15) of monoclonal growth cell wells. Clone B (SCLC-2) cells are spindle-shaped or polygonal, loosely arranged, with irregular boundaries, accounting for about 87% (13/15) of monoclonal growth cell wells.

After cloning A, cell division and proliferation are vigorous, and the wall of the culture flask can be covered in 3-4 days, and the continuous passage reaches more than 20 passages for 3 months. It takes more than 1 week for clonal B to cover the wall of the flask, and after 6-8 passages, the cells are rough, and golden particles or brown flocculent are seen on the surface.
The addition of MTT thiazolan to detect cell proliferation inhibition rate conclusion: sorafenib did not significantly change the inhibitory rate of clonal A cells, and sorafenib had obvious changes in the inhibition rate of cloned B cells.

In general, laboratories rely on manual observation to record cell growth, and experimenters are required to regularly take out the cells in culture and put them under a microscope for observation and record the corresponding data. Once the number of control groups increases, it is difficult for manual personnel to record multiple groups and record accurate data simultaneously. Moreover, the operation of frequently taking out cells and observing them artificially can easily lead to cell contamination and waste the previous achievements of research.
Therefore, in order to solve the above practical problems, the R&D team of Lixian Intelligent has comprehensively developed such a cell intelligent monitoring assistant - Cellaview. Its core advantage is that it can intelligently monitor and record cell growth status in real time 24/7, and because the volume is small enough, it can be directly put into the incubator with the dish, and it can be observed while cultured, without worrying about cell contamination caused by frequent handling.

Taking the experiment on the inhibitory effect of colchicine on the proliferation of HeLa cells as an example (30ng/ml concentration colchicine), Cellaview was used as the following control experiment. The normally cultured HeLa cells were control group, undosed group (Hela cells treated with an equal volume of 30 ng/mLNacl for 12 h), dosing group (Hela cells treated with 30 ng/ml colchicine for 12 h), and then placed in the incubator under 95% CO₂, 5% O₂, 37°C.

Experimental conclusion: Colchicine has a significant inhibitory effect on the proliferation of HeLa cells, and further comparative experiments are needed to further explore the degree of inhibition of different concentrations of colchicine on the proliferation of HeLa cells.

As above, Cellaview meets the needs of multi-group, synchronous and retainerable data in the control group experiment. Cellaview's PC background can also support the synchronous connection of up to 4 instruments, which can be applied to the type of experiments that affect the same kind of cells under different factors and the effects of the same factors on different cells, which can provide multiple sets of synchronous control reference for experiments, which is of great help to cell experimental research.

Cellaview is perfectly suited for most cell growth studies such as cell scratching, confluency recognition, organoid culture monitoring, tumor spheroid proliferation monitoring, embryonic stem cell growth monitoring, etc., which greatly helps in cell quality control and monitoring.